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Biology

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Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
 

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Article DOI: 10.3791/51060-v • 08:20 min • December 20th, 2013
December 20th, 2013 •
1, 1, 1, 1
1Department of Oncological Sciences, University of Utah

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Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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Keywords: Zebrafish Embryos Apoptosis Whole-mount Immunofluorescence Activated Caspase 3 TUNEL Assay Intrinsic Apoptosis Extrinsic Apoptosis Tissue Specificity Cell Types Fixed Embryos Mutants Morphants Overexpression Chemicals Acridine Orange Cost-effective Reliable
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