Journal
/
Biology
/
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

DOI:
10.3791/51074-v

•

11:43 min

•

May 10, 2014

•

, , , ,
1Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center,Aarhus University, 2Departments of Chemistry and Physics,McGill University

Chapters

  • 00:05Title
  • 01:15Import the Image Sequence of Interest into an Imaging Analysis Program
  • 02:37Perform the Analysis
  • 06:52Calculate Average of the Diffusion Coefficient
  • 07:56Results: Example ROI Crops, Diffusion, and k2 Plots
  • 11:09Conclusion

Summary

Automatic Translation

Automatic Translation

This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.

Tags

Diffusion CoefficientsEGFPPlasma Membrane ProteinsK-Space Image Correlation Spectroscopy (kICS)Fluorescence MicroscopyImage AnalysisAquaporin-3Membrane Protein Diffusion

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51074?article_type=v

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
check_url/51074?article_type=v

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
check_url/51074?article_type=v

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
check_url/51074?article_type=v

Crystallization of Membrane Proteins in Lipidic Mesophases
check_url/51074?article_type=v

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
check_url/51074?article_type=v

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
check_url/51074?article_type=v

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
check_url/51074?article_type=v

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
check_url/51074?article_type=v

Imaging Plasma Membrane Deformations With pTIRFM
check_url/51074?article_type=v

Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens

Read Article