Journal
/
Medicine
/
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry

Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry

DOI:
10.3791/51088-v

•

12:25 min

•

January 07, 2014

•

, , , , , , , ,
1Department of Neurosurgery,Henry Ford Hospital

Chapters

  • 00:05Title
  • 02:38Single Cell GBM (Glioblastoma) Suspension for Neurosphere Culture
  • 05:13Morphological and Molecular Analysis of Neurospheres by Immunohistochemistry
  • 08:35Results: Representative Xenograft Tumor Formation from High Grade Glioma Cell Cultures
  • 11:12Conclusion

Summary

Automatic Translation

Automatic Translation

Protocol for propagation of dissociated high grade glioma surgical specimens in serum-free neurosphere medium to select for cells with cancer stem cell phenotype. For specimens that fail to grow as neurospheres, an alternative protocol is suggested. A paraffin embedding technique for maintaining the 3D neurosphere architecture for immunocytochemistry is described.

Tags

Keywords: GlioblastomaHigh-grade AstrocytomaCell CultureNeurosphere AssayCancer Stem CellsOrthotopic Xenograft3D ImmunochemistryPreclinical Models

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51088?article_type=v

Implantation of Radiotelemetry Transmitters Yielding Data on ECG, Heart Rate, Core Body Temperature and Activity in Free-moving Laboratory Mice
check_url/51088?article_type=v

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
check_url/51088?article_type=v

Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
check_url/51088?article_type=v

An Isolated Working Heart System for Large Animal Models
check_url/51088?article_type=v

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
check_url/51088?article_type=v

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring
check_url/51088?article_type=v

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
check_url/51088?article_type=v

Surgical Models of Gastroesophageal Reflux with Mice
check_url/51088?article_type=v

Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
check_url/51088?article_type=v

A Model to Simulate Clinically Relevant Hypoxia in Humans

Read Article