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Chapters
- 00:05Title
- 01:19Dissection of the neural Crest (NC) Explants
- 03:54Plating Explants on Fibronectin for in vitro Migration Imaging
- 05:03Grafting Explants into Host Embryos for in vivo Migration Analysis
- 06:25Results: In vitro and in vivo Behavior of Explanted Cranial NC
- 08:21Conclusion
This protocol describes how to dissect premigratory cranial neural crest (NC) from Xenopus laevis neurulas. These explants can be plated on fibronectin and cultured in vitro, or grafted back into host embryos. This technique allows studying the mechanisms of NC epithelium-to-mesenchyme transition, migration, and differentiation.
