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Hurtig og effektiv zebrafisk Genotypebestemmelse Brug PCR med høj opløsning Melt Analyse
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Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Hurtig og effektiv zebrafisk Genotypebestemmelse Brug PCR med høj opløsning Melt Analyse

DOI:
10.3791/51138-v

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06:30 min

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February 05, 2014

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, , , ,
1Division of Pediatric Neurology, Department of Pediatrics,University of Utah School of Medicine, 2Department of Neurobiology and Anatomy,University of Utah School of Medicine, 3Interdepartmental Program in Neurosciences,University of Utah School of Medicine, 4Mutation Generation and Detection Core, HSC Core Research Facility,University of Utah School of Medicine, 5Department of Neurology,University of Utah School of Medicine

Chapters

  • 00:05Title
  • 01:09DNA Preparation
  • 02:24PCR Settings
  • 03:24PCR Analysis by HRMA
  • 04:31PCR Identified Mutations
  • 06:06Conclusion

Summary

Automatic Translation

Automatic Translation

PCR kombineret med høj opløsning smelte analyse (HRMA), er dokumenteret som en hurtig og effektiv metode til at genotype zebrafisk.

Tags

Keywords: ZebrafishGenotypingPCRHigh-resolution Melt AnalysisHRMAGenetic ToolsMutationTransgenicScreeningModel SystemDevelopmentDiseaseDrug ScreeningRapidEfficientSensitiveInexpensiveContamination

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