A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:48Preparation of Whole Cell Lysates from Cell Culture
- 04:00Sample Preparation and Electrophoresis
- 05:50Protein Transfer Using the iBlot Dry Blotting System
- 07:08Infrared Fluorescent Protein Detection
- 09:14Results: Improved Western Blot Sensitivity, Quality, and Accuracy
- 11:13Conclusion
This protocol explores the latest advancements in performing Western blot analyses. These novel modifications employ a Bis-Tris gel system with a 35 min electrophoresis run time, a 7 min dry blotting transfer system, and infrared fluorescent protein detection and imaging that generates higher resolution, quality, sensitivity, and improved accuracy of Western data.
