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Journal / Immunology and Infection / 2D and 3D Chromosome Painting in Malaria…
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Immunology and Infection

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JoVE Journal Immunology and Infection
2D and 3D Chromosome Painting in Malaria Mosquitoes
 

2D and 3D Chromosome Painting in Malaria Mosquitoes

Article DOI: 10.3791/51173-v • 09:57 min • January 6th, 2014
January 6th, 2014 •
1, 1, 1
1Department of Entomology, Virginia Tech

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Summary
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Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).

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Keywords: Chromosome Painting FISH Laser Capture Microdissection Whole Genome Amplification Anopheles Gambiae Malaria Mosquito Polytene Chromosome Chromosome Organization Cytogenetics
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