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Bioengineering
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使用无标记光学生物传感器由G-蛋白偶联受体,以检测钾通道的调制
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
Please note that all translations are automatically generated.
Click here for the English version.
使用无标记光学生物传感器由G-蛋白偶联受体,以检测钾通道的调制
DOI:
10.3791/51307-v
•
10:59 min
•
February 10, 2014
•
Matthew R. Fleming
,
Steven M. Shamah
,
Leonard K. Kaczmarek
2
1
Department of Pharmacology
,
Yale School of Medicine
,
2
Department of Cellular and Molecular Physiology
,
Yale School of Medicine
,
3
X-BODY Biosciences
Chapters
00:05
Title
02:30
384-well Optical Biosensor Plate Coating
03:39
Seeding of Cells onto the 384-well Optical Biosensor Plate
04:46
Resonance Wavelength Grating (RWG) Optical Biosensor Assay
06:40
Analysis of Assay Data with BIND View
07:52
Results: Analysis of Peak Wavelength Value to Detect Modulation of Potassium Channels
10:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
光学生物传感器技术可检测的变化在质量近质膜在活细胞中,并允许1到跟随在这两个单个细胞和细胞群的细胞反应。这个协议将描述检测钾通道通过使用这种方法的G蛋白偶联受体在完整的细胞调制的。
Tags
Label-free
Optical Biosensors
Potassium Channels
G-protein Coupled Receptors
Resonance Wavelength Grating
Cell Adhesion
Protein-protein Interactions
Ion Channels
GPCR
KNa Channels
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