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Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
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JoVE Journal Neuroscience
Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

DOI:
10.3791/51310-v

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10:03 min

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August 10, 2014

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, , , ,
1RG Neuroplasticity,Leibniz Institute for Neurobiology, 2Department of Cell Biology,Utrecht University

Chapters

  • 00:05Title
  • 02:03Preparation of Hippocampal Slices
  • 03:13Baseline Recording and LTP Induction
  • 05:17Isolation of Nuclear Enriched Fractions from Hippocampal CA1
  • 07:29Semiquantitative Immunoblotting for Synapto-nuclear Proteins
  • 08:02Results: Induction of CA1 LTP Leads to the Accumulation of pJacS180 in the Nucleus
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

We provide a detailed protocol for induction of long-term potentiation in the CA1 region of the hippocampus and the subsequent isolation of nuclear enriched fractions from the tetanized area of the slice. This approach can be used to determine activity dependent nuclear protein import in cellular models of learning and memory.

Tags

Keywords: Nuclear Enriched FractionHippocampal SlicesSynaptic PlasticityLong-term PotentiationLong-term DepressionProtein TranslocationImmunoblottingActivity-dependentSignaling CascadesCA1 NeuronsERK1/2Jacob

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