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Nanopodia - 薄，易碎膜预测与细胞运动和细胞间的相互作用角色

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Biology

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JoVE Journal Biology

Nanopodia – Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions

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Nanopodia - 薄，易碎膜预测与细胞运动和细胞间的相互作用角色

DOI:

10.3791/51320-v

•

10:50 min

•

April 03, 2014

•

Chi-Iou Lin, Chun-Yee Lau, Dan Li, Shou-Ching Jaminet

¹Center for Vascular Biology Research, Department of of Pathology, Beth Israel Deaconess Medical Center,Harvard Medical School

Chapters

00:05Title
01:25Cell Culture on Collagen Coated Glass Disk
03:44Cell Fixation
05:24TM4SF1 Immunofluorescence Staining of Nanopodia
07:25Results: Tracking the Nanopodia
10:07Conclusion

Summary

Automatic Translation

Nanopodia很瘦但很脆弱的膜通道，向上延伸至100微米的细胞的领先的前面或后面尾随和检测的细胞环境。直接固定在较高的Triton X-100浓度为37℃，轻柔洗涤，并避免如乙醇，甲醇，丙酮或有机溶剂，并须遵守这些细胞结构。

Tags

Nanopodia Thin Membrane Projections Cell Movement Intercellular Interactions TM4SF1 TMED F-actin Endothelial Cells Cancer Cells Cell Polarization Cell Signaling Cell-cell Communication

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