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Chapters
- 00:05Title
- 02:01Determine Intracellular Ascorbate in Cultured Cells
- 04:16Determine the Amount of Ascorbate Released and Extracellular Ascorbate
- 06:25Results: Determination of Intracellular and Extracellular Ascorbate in Cultured Cells
- 11:36Conclusion
Ascorbate plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. Here we describe a medium-throughput, specific and inexpensive microplate assay for the determination of both intra- and extracellular ascorbate in cell culture.
