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Protein-protein interaktioner visualiseret ved bimolekylær Fluorescens komplementering i tobaksprotoplaster og blade
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JoVE Journal Biology
Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

Protein-protein interaktioner visualiseret ved bimolekylær Fluorescens komplementering i tobaksprotoplaster og blade

DOI:
10.3791/51327-v

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11:10 min

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March 09, 2014

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1Department Biologie I, Botanik,Ludwig-Maximilians-Universität, München

Chapters

  • 00:05Title
  • 01:18Transient Transformation of Tobacco Leaves
  • 02:59Isolation of Protoplasts
  • 04:52Laser Scanning Microscopy
  • 07:18Results: BiFC Enables Visualization of Protein-protein Interactions in Tobacco Leaf Cells
  • 10:45Conclusion

Summary

Automatic Translation

Automatic Translation

Dannelse af protein-komplekser in vivo kan visualiseres ved bimolekylær fluorescens komplementering. Interaktionspartnere fusioneres til komplementære dele af fluorescerende tags og udtrykt transient i tobaksblade, hvilket resulterer i et rekonstitueret fluorescenssignal ved tæt nærhed af de to proteiner.

Tags

Bimolecular Fluorescence Complementation (BiFC)Protein-protein InteractionsTobacco Protoplasts And LeavesIn Vivo VisualizationSubcellular LocalizationMarker GenesConfocal MicroscopyAgrobacterium-mediated TransformationLimitations Of BiFC

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