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Glycopeptid Capture for Cell Surface Proteomics
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JoVE Journal Biology
Glycopeptide Capture for Cell Surface Proteomics

Glycopeptid Capture for Cell Surface Proteomics

DOI:
10.3791/51349-v

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10:11 min

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May 09, 2014

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1Department of Chemistry,Simon Fraser University

Chapters

  • 00:05Title
  • 01:37Harvest Membranes
  • 02:54Dissolve, Denature, and Digest Membrane Proteins
  • 05:34Glycopeptide Capture
  • 08:04Results: LC-MS Based Proteomic Analysis of Cell Surface Proteins
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Celleoverfladeproteiner er biologisk vigtige og almindeligt glycosyleret. Vi introducerer her en glycopeptid-capture metode til at opløse, berige og deglycosylate disse proteiner for letkøbte LC-MS baserede proteomikanalyser.

Tags

Glycopeptide CaptureCell Surface ProteomicsN-glycoproteomeSurface ProteinsBiomarker DiscoveryCell-type IdentificationDrug-target SelectionCellular BiologyCellular FunctionsPost-translational ModificationsLC-MSCancerStem CellsDrug Toxicity

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