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Bio-lags Interferometry til måling Kinetik af protein-protein interaktioner og allosteriske Ligand Effects
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
DOI:

13:57 min

February 18, 2014

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Chapters

  • 00:05Title
  • 02:05Programming the Instrument for BLI Assay
  • 05:44Sample Preparation
  • 07:52Data Processing and Analysis
  • 10:40Results: Analysis of BLI Kinetics to Determine Rate Constants and Binding Affinity
  • 13:14Conclusion

Summary

Automatic Translation

Protokollerne her beskriver kinetiske analyser af protein-protein interaktioner med Bio-lags Interferometry. F-typen ATP syntase, som er involveret i cellulære energi metabolisme, kan hæmmes af sin ε underenhed i bakterier. Vi har tilpasset Bio-lags interferometri at studere interaktioner af det katalytiske kompleks med ε hæmmende C-terminale domæne.

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