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Wholemount 쥐의 망막에서 면역 조직 화학 및 칼슘 이미징 방법
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Neuroscience
Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
Please note that all translations are automatically generated.
Click here for the English version.
Wholemount 쥐의 망막에서 면역 조직 화학 및 칼슘 이미징 방법
DOI:
10.3791/51396-v
•
08:54 min
•
October 13, 2014
•
Allison Sargoy
,
Steven Barnes
2,3
,
Nicholas C. Brecha
2,4
,
Luis Pérez De Sevilla Müller
1
Department of Neurobiology
,
University of California, Los Angeles
,
2
Veterans Administration Greater Los Angeles Healthcare System
,
3
Departments of Physiology & Biophysics and Ophthalmology & Visual Sciences
,
Dalhousie University
,
4
Departments of Neurobiology and Medicine, Jules Stein Eye Institute, CURE-Digestive Diseases Research Center, David Geffen School of Medicine
,
University of California, Los Angeles
Chapters
00:05
Title
01:25
Eyecup Preparation
02:58
Wholemount Retina Preparation and Processing
05:06
Calcium Imaging in Retinal Ganglion Cells
07:19
Results: RGC Immunostaining and Electrophysiology
08:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
면역 프로토콜은 망막에있는 특정 단백질의 현지화를 연구하는 데 사용됩니다. 칼슘 이미징 기술은 망막 신경절 세포 축색 돌기 칼슘 역학을 연구하기 위해 사용된다.
Tags
Keywords: Immunohistochemistry
Calcium Imaging
Wholemount Retina
Retinal Ganglion Cells
Voltage-gated Calcium Channels
Displaced Amacrine Cells
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