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Chapters
- 00:05Title
- 01:40Lysate Preparation
- 02:29Removal of Free c-di-GMP and Other Nucleotides – Soluble Fraction Only
- 03:08Pellet Resuspension and Solubilization – Membrane Fraction Only
- 05:34Washing Steps and MS Sample Preparation
- 09:42Results: Capture Compound Mass Spectrometric Identification of c-di-GMP Effector Proteins from P. aeruginosa
- 11:30Conclusion
The ubiquitous second messenger c-di-GMP controls growth and behavior of many bacteria. We have developed a novel Capture Compound Mass Spectrometry based technology to biochemically identify and characterize c-di-GMP binding proteins in virtually any bacterial species.
