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两种方法从子宫切除标本小学建立人子宫内膜基质细胞
JoVE Journal
Medicine
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JoVE Journal
Medicine
Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Please note that all translations are automatically generated.
Click here for the English version.
两种方法从子宫切除标本小学建立人子宫内膜基质细胞
DOI:
10.3791/51513-v
•
09:15 min
•
May 23, 2014
•
Kasey Jividen*
1
,
Mercedeh Javanbakht Movassagh*
1
,
Amir Jazaeri
,
Hui Li
1
Department of Pathology
,
University of Virginia
,
2
Department of Obstetrics & Gynecology
,
University of Virginia
Chapters
00:05
Title
01:34
Prepariation of Primary Cell Lines using the Scraping Method
04:56
Preparation of Primary Cell Lines using the Trypsin Method
07:07
Results: Establishment of an Endometrial Stromal Culture
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
建立基层子宫内膜间质细胞培养系统从子宫切除标本是追求研究的目的繁多前一个有价值的生物技术和关键的一步。在这里,我们描述了两种方法用于建立从人类患者手术切除的子宫内膜组织间质文化。
Tags
Primary Human Endometrial Stromal Cells
In Vitro Cell Culture
Hysterectomy Specimens
Scraping Method
Trypsin Method
Endometrial Pathologies
Gynecological Cancers
Infectious Diseases
Reproductive Deficiencies
Quantitative Real-time PCR
Immunofluorescence
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