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Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
 
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Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens

Article DOI: 10.3791/51513-v 09:15 min May 23rd, 2014
May 23rd, 2014

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Summary

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Click here for the English version.

Establishing primary endometrial stromal cell culture systems from hysterectomy specimens is a valuable biological technique and a crucial step prior to pursuing a vast array of research aims. Here, we describe two methods used to establish stromal cultures from surgically resected endometrial tissues of human patients. 

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Keywords: Primary Human Endometrial Stromal Cells In Vitro Cell Culture Hysterectomy Specimens Scraping Method Trypsin Method Endometrial Pathologies Gynecological Cancers Infectious Diseases Reproductive Deficiencies Quantitative Real-time PCR Immunofluorescence
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