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Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva
 

Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

Article DOI: 10.3791/51595-v • 08:22 min • June 17th, 2014
June 17th, 2014 •
1, 1
1DFG-Center for Regenerative Therapies Dresden, Technische Universität Dresden

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Summary
Automatic Translation

An adaptable reverse genetic method for zebrafish to assess gene function during later stages of development and physiological homeostasis such as tissue regeneration using intraventricular injections of gene-specific morpholinos.

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Keywords: Zebrafish Reverse Genetics Morpholino Cardiac Ventricular Injection Larval Tissue Gene Function Tissue Regeneration Tissue Homeostasis Embryogenesis Finfold Regeneration Vertebrate Regeneration
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