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Chapters
- 00:05Title
- 02:10Isolation of Arabidopsis Mesophyll Protoplasts
- 04:13The Hypotonic Challenge Assay: Arabidopsis Mesophyll Cell Swelling
- 06:36Analysis of Cell Volume Change Using ImageJ
- 08:12Modling the Rate of Osmolarity Change in the Experimental Chamber Using ImageJ and the Matlab Program PfFit
- 11:11Determining the Pf Using the Matlab Fitting Program PfFit
- 12:30Results: Determination of the Osmotic Water Permeability Coefficient (Pf) in Plant Protoplasts
- 13:37Conclusion
Måling af osmotiske koefficient vandpermeabiliteten (P f) af celler kan hjælpe med at forstå de regulatoriske mekanismer aquaporiner (AQPs). P f beslutsomhed i sfæriske plantecelleprotoplaster præsenteres her involverer protoplaster isolation og numerisk analyse af deres oprindelige sats på volumen forandring som følge af en osmotisk udfordring under konstant bad perfusion.
