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在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

JoVE Journal
Biology

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JoVE Journal Biology

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Please note that all translations are automatically generated. Click here for the English version.

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

DOI:

10.3791/51683-v

•

12:54 min

•

September 04, 2014

•

Mark K. Scott, Olufolake Akinduro, Cristina Lo Celso

¹Department of Life Science and Facility for Imaging by Light Microscopy,Imperial College London, ²Department of Life Sciences,Imperial College London

Chapters

00:05Title
01:52Labeling and Injection of HSPCs
03:21Preparing the Mouse for Imaging
05:20In Vivo Imaging: High-resolution Stacks and Time-lapse Acquisition
11:25Results: In Vivo 4D Imaging of HSPCs in Mouse Calvarium
12:16Conclusion

Summary

Automatic Translation

造血干细胞和祖细胞（HSPCs）和骨髓龛之间的相互作用的性质知之甚少。定制的硬件修改和多步获取协议允许使用双光子共焦显微术图像体外骨髓区域内驻留标记HSPCs，跟踪相互作用和运动。

Tags

Hematopoietic Stem Cells HSCs Hematopoietic Stem And Progenitor Cells HSPCs Bone Marrow Microenvironment In Vivo Imaging 4-dimensional Tracking Confocal Imaging Two-photon Imaging Osteoblastic Cells

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