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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
 

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Article DOI: 10.3791/51712-v • 09:19 min • July 22nd, 2014
July 22nd, 2014 •
*1,2, *1,2, 1,2,3
1Department of Biochemistry, McGill University, 2Institute of Parasitology, McGill University, 3McGill Centre for Bioinformatics, McGill University
* These authors contributed equally

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Summary
Automatic Translation

A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as therapeutics.

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Keywords: RNA Editing Trypanosomes Editosome Fluorescence-based Reporter Assay FRET High-throughput Screening Chemical Compounds Molecular Probes In Vitro RNA Editing
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