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Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
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1Department of Molecular Biology and Genetics,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 2Department of Neuroscience,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 3Department of Ophthalmology,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine
Chapters
- 00:06Title
- 02:01Dorsal Skin for Immunostaining and Melanin Imaging
- 05:40Foot Skin for the Analysis of Hair Follicle Patterning
- 06:42Foot Skin for Visualizing Sebaceous Glands
- 07:23Tail Skin Preparation for the Analysis of Melanin Distribution, Hair Follicle Orientation, and Sebaceous Gland Visualization
- 08:54Imaging Hair Follicle Orientation
- 09:52Imaging and Tracing Axon Arbors
- 10:55Results: Geometry of Various Skin Structures Are Easily Visualized in Skin Flatmounts
- 12:51Conclusion
Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.
