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Journal / Neuroscience / Primary Culture of Mouse Dopaminergic Neurons
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Neuroscience

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JoVE Journal Neuroscience
Primary Culture of Mouse Dopaminergic Neurons
 

Primary Culture of Mouse Dopaminergic Neurons

Article DOI: 10.3791/51751-v • 11:58 min • September 8th, 2014
September 8th, 2014 •
1,2,3, 1,2,3, 1,2,3
1CNRS, UMR-5203, Institut de Génomique Fonctionnelle, Montpellier, 2Inserm, U661, Montpellier, 3Universités de Montpellier

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Summary
Automatic Translation

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.

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Keywords: Primary Culture Mouse Dopaminergic Neurons Parkinson's Disease Nigrostriatal Neurons Neurodegeneration Ventral Mesencephalon Transgenic Mouse Models Immunofluorescence QPCR Second Messengers Neuronal Death/survival
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