Journal
/
/
酵母核内体系统的超微结构分析的纳米金标记
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
DOI:

09:49 min

July 14, 2014

, ,

Chapters

  • 00:05Title
  • 01:21Spheroplast Preparation
  • 03:07Nanogold Uptake
  • 03:44Fixation and Sectioning
  • 04:42Silver Enhancement for Nanogold Particle Visualization
  • 08:17Results: Yeast Endosomal Compartments are Visualized by Silver-enhanced Nanogold Labeling
  • 09:09Conclusion

Summary

Automatic Translation

酵母, 酿酒酵母 ,一直是一个关键模式生物识别和研究基因调节内体系统的生物合成和功能。在这里,我们提出了内体车厢的超微结构研究特定的标签一个详细的协议。

Related Videos

Read Article