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Dyrkning<em> Caenorhabditis elegans</em > I Axeniske Liquid Media og fremstilling af transgene Worms ved mikropartikelbombardement
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Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment

Dyrkning<em> Caenorhabditis elegans</em > I Axeniske Liquid Media og fremstilling af transgene Worms ved mikropartikelbombardement

DOI:
10.3791/51796-v

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08:26 min

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August 02, 2014

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, , ,
1Department of Animal and Avian Sciences,University of Maryland, 2Department of Cell Biology and Molecular Genetics,University of Maryland

Chapters

  • 00:05Title
  • 01:26Preparation of Modified C. elegans Habituation and Reproduction Medium (mCeHR)
  • 02:50Preparing C. elegans for mCeHR Culture
  • 05:39Synchronizing and Storing Worms in mCeHR Culture
  • 06:55Results: Nutrient Studies and Trangene Delivery in mCeHR
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

C. elegans er normalt dyrkes på faste agarplader eller i flydende kulturer podet med E. coli. At forebygge bakterielle biprodukter fra confounding toksikologiske og ernæringsmæssige undersøgelser vi udnyttet en aksenisk flydende medium, CeHR, at vokse og synkronisere et stort antal orme for en række efterfølgende anvendelser.

Tags

Caenorhabditis ElegansAxenic Liquid MediaMCeHR MediaMicroparticle BombardmentTransgenic WormsNutrient RequirementsHeme ConcentrationFluorescent SensorsGrowth And DevelopmentToxicological Effects

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