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一种新型的<em>在体内</em>基因转移技术和<em>在体外</em>细胞分析为肌肉骨骼疾病骨质流失的研究
JoVE Journal
Medicine
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JoVE Journal Medicine
A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
DOI:

11:47 min

June 08, 2014

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Chapters

  • 00:00Title
  • 00:39Hydrodynamic delivery of RANKL MC DNA
  • 03:04In vitro osteoclast generation from mouse BMM
  • 05:42In vitro osteoclast generation from human PBMC
  • 09:31Results

Summary

Automatic Translation

Differentiation of precursor cells into osteoclasts is regulated by cytokines and growth factors. Here, a novel gene transfer technique for differentiation of osteoclasts in vivo and cell culture protocols for differentiating precursor cells into osteoclasts in vitro as a method to study the effects of cytokines on osteoclastogenesis are described.

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