Journal
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Analyse af Tubular Membrane Networks i hjertemyocytter fra Atria og hjertekamre
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
DOI:

10:30 min

October 15, 2014

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Chapters

  • 00:05Title
  • 01:46Isolation of Atrial and Ventricular Myocytes from the Mouse Heart
  • 04:09Imaging of Transverse-axial Tubule System (TATS) Membrane Structures in Living Myocytes
  • 05:41Analysis of the TATS Membrane Network and its Components
  • 08:23Results: Representative TATS Component Orientations for Ventricular and Atrial Myocytes
  • 09:26Conclusion

Summary

Automatic Translation

I hjertemyocytter, rørformede membran strukturer danner intracellulære netværk. Vi beskriver optimerede protokoller for i) isolering af myocytter fra mus hjerte, herunder kvalitetskontrol, ii) levende celler farvning for state-of-the-art fluorescens mikroskopi, og iii) direkte billede analyse til beregning af komplekse komponenter og plasticitet intracellulær membran netværk.

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