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Multimodale Optisk mikroskopi Metoder Reveal Polyp vævsmorfologi og struktur i Caribien Reef Building Koraller
JoVE Journal
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JoVE Journal Environment
Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
DOI:

10:39 min

September 05, 2014

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Chapters

  • 00:05Title
  • 01:04Wax Preinfiltration and Embedding
  • 06:18Imaging Corals Under Two-photon Fluorescence Microscopy
  • 07:293D Volume Rendering and Visualization of SBFI and Two-photon Spectral Fluorescence Data
  • 08:55Results: Two-photon Fluorescence 3D Images of Coral Polyps
  • 09:58Conclusion

Summary

Automatic Translation

En integreret suite af billeddannende teknikker er blevet anvendt til at bestemme polyp morfologi og væv struktur i Caribien koraller Montastraea annularis og M. faveolata. Fluorescens, seriel blok ansigt, og to-foton konfokal laser scanning mikroskopi har identificeret tunget struktur, polyp væggene, og estimeret chromatophore og zooxanthellae tætheder og fordelinger.

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