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Ex vivo Kultur av Drosophila PUPP Testikel och Single Man Germ linjer Cystor: Dissection, Imaging och Farmakologisk behandling
JoVE Journal
Biology
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JoVE Journal Biology
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
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Ex vivo Kultur av Drosophila PUPP Testikel och Single Man Germ linjer Cystor: Dissection, Imaging och Farmakologisk behandling

DOI:
10.3791/51868-v

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08:35 min

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September 11, 2014

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, , ,
1Fachbereich Biologie, Entwicklungsbiologie,Philipps-Universität Marburg, 2Institut für Molekularbiologie und Tumorforschung,Philipps-Universität Marburg

Chapters

  • 00:05Title
  • 01:12Dissection of Pupal Testes
  • 03:40Dissection of Male Germ-line Cysts and Live Imaging
  • 04:58Pharmacological Assay with Intact Pupal Testes
  • 06:30Results: Dynamic Analysis of Drosophila Testes
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

Tags

DrosophilaPupal TestisEx Vivo CultureGerm-line CystsSpermatogenesisPost-meioticHistone-to-protamine SwitchPharmacological TreatmentMicroscopic Imaging

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