Journal
/
Biology
/
Ikke-enzymatisk, Serumfrit Tissue Culture of Pre-invasive brystlæsioner til spontane generation af Mammospheres
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

Ikke-enzymatisk, Serumfrit Tissue Culture of Pre-invasive brystlæsioner til spontane generation af Mammospheres

DOI:
10.3791/51926-v

•

09:49 min

•

November 08, 2014

•

, ,
1Center for Applied Proteomics and Molecular Medicine,George Mason University, 2Virginia Surgery Associates

Chapters

  • 00:05Title
  • 02:03Tissue Acquisition and Grossing
  • 04:22Maintenance of Established Organoid/Epithelial Cell Colonies
  • 06:43Results: Representative Mammosphere Imaging
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Primær cellekultur anvendelse af intakte væv organoids tilvejebringer en model, der efterligner den flercellede in vivo mikromiljø. Vi udviklede en serum-frit primær brystepitel vævskultur model, der foreviger blandede cellekultur slægter og udstillinger differentierede morfologi, uden enzymatisk vævssprængning. Breast organoids forbliver levedygtige i> 6 måneder.

Tags

Non-enzymaticSerum-freePre-invasive Breast LesionsSpontaneous GenerationMammospheresBreast Ductal Carcinoma In Situ (DCIS)Progenitor CellsInvasionOrganoidsEpithelial CellsFibroblastsCytogenetically AbnormalInvasive Progenitor Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51926?article_type=v

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
check_url/51926?article_type=v

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
check_url/51926?article_type=v

Generation of Single-Cell Suspensions from Mouse Neural Tissue
check_url/51926?article_type=v

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
check_url/51926?article_type=v

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
check_url/51926?article_type=v

Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
check_url/51926?article_type=v

Kvantitativ sammenligning af Cis-Regulatory Element (CRE) Aktiviteter i transgene Drosophila melanogaster</em
check_url/51926?article_type=v

Mus embryonale udvikling i et serum-frit Hele Embryo Culture System
check_url/51926?article_type=v

Generation og Kultur of Blood Udvækst endotelceller fra human perifert blod
check_url/51926?article_type=v

En vævskultur Model af østrogen-producerende primære Bovine Granulosa-celler

Read Article