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Ikke-enzymatisk, Serum-free Tissue Culture of Pre-invasive brystlesjonene for Spontan generasjon Mammospheres
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JoVE Journal Biology
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

Ikke-enzymatisk, Serum-free Tissue Culture of Pre-invasive brystlesjonene for Spontan generasjon Mammospheres

DOI:
10.3791/51926-v

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09:49 min

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November 08, 2014

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, ,
1Center for Applied Proteomics and Molecular Medicine,George Mason University, 2Virginia Surgery Associates

Chapters

  • 00:05Title
  • 02:03Tissue Acquisition and Grossing
  • 04:22Maintenance of Established Organoid/Epithelial Cell Colonies
  • 06:43Results: Representative Mammosphere Imaging
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Primære cellekultur ved hjelp av intakte vev organoids gir et modellsystem som etterligner multi-mobilnettet in vivo mikromiljøet. Vi utviklet et serum-free primære brystepitel vevskultur modell som foreviger blandede cellekultur linjene og utstillinger differensierte morfologi, uten enzymatisk vev avbrudd. Bryst organoids være levedyktig i> 6 måneder.

Tags

Non-enzymaticSerum-freePre-invasive Breast LesionsSpontaneous GenerationMammospheresBreast Ductal Carcinoma In Situ (DCIS)Progenitor CellsInvasionOrganoidsEpithelial CellsFibroblastsCytogenetically AbnormalInvasive Progenitor Cells

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