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In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

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Biology

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JoVE Journal Biology

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

DOI:

10.3791/51943-v

•

10:07 min

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November 13, 2014

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Meltem Avci-Adali, Andreas Behring, Heidrun Steinle, Timea Keller, Stefanie Krajeweski, Christian Schlensak, Hans P. Wendel

¹Department of Thoracic, Cardiac, and Vascular Surgery,University Hospital Tuebingen

Chapters

00:05Title
01:35Amplification of Plasmid Inserts and Addition of Poly T-tail by PCR
02:48In Vitro Transcription
03:59Treatment of Purified mRNA with Antartic Phosphatase
05:17mRNA Transfection of Cells
07:01Flow Cytometric Analyses of eGFP Expression in Cells
08:14Results: Modified mRNA is Expressed in Human Cells
09:34Conclusion

Summary

Automatic Translation

In this video article, we describe the in vitro synthesis of modified mRNA for induction of protein expression in cells.

Tags

MRNA Synthesis In Vitro Transcription Modified MRNA Protein Expression Transfection HEK293 Cells EGFP Regenerative Medicine Cell Biology Reprogramming

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