Journal
/
/
Utilizing Custom-designed Galvanotaxis Chambers to Study Directional Migration of Prostate Cells
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Utilizing Custom-designed Galvanotaxis Chambers to Study Directional Migration of Prostate Cells
DOI:

08:45 min

December 07, 2014

, ,

Chapters

  • 00:05Title
  • 01:11Assembling the Galvanotaxis Chamber and Agar Bridges
  • 05:37Time-lapse Imaging
  • 06:58Quantification of Galvanotaxis
  • 07:41Results: Representative Galvanotaxis of Prostate Cells
  • 08:24Conclusion

Summary

Automatic Translation

We present a method to apply a physiological electric field to migrating, immortalized prostate cells in a custom-made galvanotaxis chamber. Using this method, we demonstrate that 2 lines of non-tumorigenic prostate cells demonstrate different degrees of migration directionality in the field.

Related Videos

Read Article