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JoVE Journal
Immunology and Infection

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Measuring Local Anaphylaxis in Mice
 

Measuring Local Anaphylaxis in Mice

Article DOI: 10.3791/52005-v 07:49 min October 14th, 2014
October 14th, 2014

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Summary

Allergic responses, characterized by the activation of mast cells and basophils, are driven by the cross-linking of IgE and release of proinflammatory mediators. A quantitative assessment of allergic responses can be achieved by using Evans Blue dye to monitor changes in vascular permeability after allergen challenge.

Tags

Keywords: Local Anaphylaxis Allergic Response Mast Cells Basophils Vascular Permeability OVA Sensitization Evans Blue Dye Spectrophotometry
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