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Non-invasive Model of Neuropathogenic<em> Escherichia coli</em> Infektion i Neonatal Rat
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

Non-invasive Model of Neuropathogenic<em> Escherichia coli</em> Infektion i Neonatal Rat

DOI:
10.3791/52018-v

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08:27 min

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October 29, 2014

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, , , ,
1School of Pharmacy,University College London, 2Mucin Biology Group,University of Gothenburg

Chapters

  • 00:05Title
  • 01:14E. coli K1 Feeding and Disease Severity Assessment
  • 03:27Collecting the GI Tract for Downstream Analysis
  • 05:44Tissue Processing and Homogenization
  • 06:31Results: E. coli K1 Colonization Mimics Natural Infection in Humans
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

Her er en fremgangsmåde beskrevet til etablering af systemisk infektion i nyfødte rotter med kulturer af Escherichia coli K1. Denne ikke-invasiv procedure tillader kolonisering af mavetarmkanalen, translokation af patogenet til den systemiske cirkulation, og invasion af det centrale nervesystem ved choroid plexus.

Tags

Neuropathogenic Escherichia ColiNeonatal RatNon-invasive Infection ModelGut-lymph-blood-brain InfectionAge-dependent InfectionE. Coli O18:K1VirulenceMeningeal InvasionCentral Nervous System InfectionPathogenesisTherapeutic InterventionsBacterial Virulence

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