Journal
/
Biology
/
Kvantitativ immunofluorescensassay at måle variationen i proteinniveauer på centrosomer
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Kvantitativ immunofluorescensassay at måle variationen i proteinniveauer på centrosomer

DOI:
10.3791/52030-v

•

09:39 min

•

December 20, 2014

•

,
1Department of Molecular Genetics,The Ohio State University

Chapters

  • 00:05Title
  • 02:13Cell Growth and Treatment
  • 03:26Immunostaining
  • 06:28Immunofluorescence Imaging and Analysis: Centrosome Distance < 2 μm
  • 07:58Results: Centrosomal VDAC3 Regulated by Proteasome Mediated Degradation
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a novel quantitative fluorescence assay is developed to measure changes in the level of a protein specifically at centrosomes by normalizing that protein’s fluorescence intensity to that of an appropriate internal standard.

Tags

Keywords: Quantitative ImmunofluorescenceProtein LevelsCentrosomesMitotic SpindlePrimary CiliaCell CycleCentriole AssemblyFluorescence MicroscopyVDAC3Proteasome-mediated Degradation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52030?article_type=v

Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells
check_url/52030?article_type=v

Den MultiBac Protein Complex Production Platform på EMBL
check_url/52030?article_type=v

Flow cytometri af bimolekylær Fluorescens Complementation: En High Throughput Kvantitativ metode til at studere protein-protein interaktion
check_url/52030?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/52030?article_type=v

Imaging centrosomes i Fly Testiklerne
check_url/52030?article_type=v

Måling af hæmoglobinsyntese Niveauer i pattedyrceller
check_url/52030?article_type=v

In vitro Methylering Assay til at studere protein Arginin Methylering
check_url/52030?article_type=v

Grønt fluorescerende protein-baserede Expression Screening membranproteiner i<em> Escherichia coli</em
check_url/52030?article_type=v

In vitro assay til måling Phosphatidylethanolamin methyltransferaseaktivitet
check_url/52030?article_type=v

Brug mikrofluidenheder til måling Levetid og cellefænotyper i Single Spirende gærceller

Read Article