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Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
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Bioengineering
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JoVE Journal Bioengineering
Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution

DOI:
10.3791/52186-v

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08:23 min

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October 31, 2014

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, , ,
1Department of Biomedical Engineering,University of Wisconsin, Madison, 2Department of Mechanical Engineering,University of Wisconsin, Madison

Chapters

  • 00:05Title
  • 01:49Surface-initiated Atom Transfer Radical Polymerization (SI-ATRP) of PEGMEMA
  • 03:15Functionalization of Micropatterned PEGMEMA Chains
  • 05:11“Click” Biotinylation of PEGMEMA Chains Including Staining
  • 07:10Results: System Accuracy
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

Cell culture substrates functionalized with microscale patterns of biological ligands have immense utility in the field of tissue engineering. Here, we demonstrate the versatile and automated manufacture of tissue culture substrates with multiple, micropatterned poly(ethylene glycol) brushes presenting orthogonal chemistries that enable spatially precise and site-specific immobilization of biological ligands.

Tags

Culture SubstratesPoly(ethylene Glycol) (PEG) BrushesMicrocontact PrintingRobotic Microcontact Printing (R-μCP)Sequential Nucleophilic SubstitutionMicroscale PatterningCell-material InteractionsBiospecific Interactions

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