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Protocol for Studying Extinction of Conditioned Fear in Naturally Cycling Female Rats
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JoVE Journal Behavior
Protocol for Studying Extinction of Conditioned Fear in Naturally Cycling Female Rats

Protocol for Studying Extinction of Conditioned Fear in Naturally Cycling Female Rats

DOI:
10.3791/52202-v

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09:07 min

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February 23, 2015

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, , , ,
1Department of Psychiatry,Massachusetts General Hospital, 2Harvard Medical School

Chapters

  • 00:05Title
  • 02:06Monitoring the Estrous Cycle
  • 03:25Pre-exposure and Habituation/Fear Conditioning
  • 05:41Estradiol Preparation and Administration, Extinction Training, and Extinction Recall
  • 07:05Results: Extinction of Conditioned Fear in Naturally Cycling Female Rats
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Gonadal hormones such as estrogen modulate memory formation in a number of experimental paradigms including fear extinction memory. This protocol describes a set of methods for investigating the influence of gonadal hormones specifically during extinction in naturally cycling females, including estrous cycle monitoring and exogenous estrogen administration.

Tags

Fear ExtinctionEstrous CycleFemale RatsGonadal HormonesEstrogenVaginal SwabbingBehavioral TasksNeural MechanismsSex DifferencesFear Conditioning

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