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1Experimental and Regenerative Neurosciences, School of Animal Biology,The University of Western Australia, 2School of Animal Biology and The Oceans Institute,The University of Western Australia, 3Experimental and Regenerative Neurosciences, School of Anatomy, Physiology and Human Biology,The University of Western Australia
Chapters
- 00:00Title
- 01:29Optical Calibration, Measuring Light Output
- 04:51Cell Preparation
- 05:22Inducing Oxidative Stress Prior to Light Therapy
- 05:54First Dosages of Light Treatment
- 06:36Final Dosage of Light Treatment and Detection of ROS
- 07:47Conclusion
Non-coherent Xenon light was passed through narrow-band interference and neutral density filters to deliver light of varying wavelength and intensity to cultured cells. This protocol was used to assess the effects of red/near-infrared light therapy on production of reactive species in vitro: no effects were observed using the tested parameters.
