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التمهيدي لالمناعية على نسيج Cryosectioned دماغ الفئران: مثال تلطيخ لالدبقية الصغيرة والخلايا العصبية
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
DOI:

07:30 min

May 12, 2015

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Chapters

  • 00:05Title
  • 01:17Slide Processing and Tissue Staining
  • 04:58Fluorescent Imaging of the Stained Tissue
  • 05:55Results: The Good and the Bad: Fluorescent Images Iba-1/Pan-neuronal Double-labelling
  • 07:03Conclusion

Summary

Automatic Translation

This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brains, using markers for microglia and neuronal elements as an example.

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