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1Department of Cell Biology,UT Southwestern Medical Center, 2National Institute of Neurological Disorders and Stroke,National Institute of Health, 3Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine,Medical College of Wisconsin, 4Division of Genetics and Genomics,Boston Children’s Hospital
Chapters
- 00:05Title
- 01:27Myogenic Cell Isolation
- 04:33Preparing the Virus Aliquots
- 06:47Immortalizing the Myogenic Cells
- 08:20Clonal Selection of Immortalized Myogenic Cells
- 09:30Results: Characterizing Immortalized Myogenic Cells
- 10:35Conclusion
This protocol describes techniques for live cell isolation and primary culture of myogenic and fibroblast cell lines from muscle or skin tissue. A technique for the immortalization of these cell lines is also described. Altogether, these protocols provide a reliable tool to generate and preserve patient-derived cells for downstream applications.
