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거대 분자 3D 재건을위한 샘플 준비에 프라이머 높은 품질 데이터 수집 : 곳을 알아내는 - 전자 현미경의 것과하지 말아야를 수행
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Do’s and Don’ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
DOI:

09:25 min

January 09, 2015

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Chapters

  • 00:05Title
  • 01:21Cryo-holder Preparation and Maintenance
  • 02:32Grid Preparation
  • 03:48Plunge-freezing
  • 04:50Transfer the Cryo-grid to the TEM
  • 06:15Low-dose Data Collection
  • 07:03Results: Visualization of Macromolecules Using Cryo-electron Microscopy
  • 08:51Conclusion

Summary

Automatic Translation

This paper describes the cryo-electron microscopy methodology, which is used to obtain high quality microscopic images of macromolecules in their near-native state. The method yields images suitable for further computerized processing using the single particle approach, devised to generate the 3D reconstruction of a macromolecule.

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