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Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
 

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

Article DOI: 10.3791/52338-v • 07:35 min • April 7th, 2015
April 7th, 2015 •
1,2, 1, 1, 1, 3, 3, 4, 5, 1,2,6
1Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 2Eck Institute for Global Health, University of Notre Dame, 3Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, 4INRS-Institut Armand-Frappier, 5Department of Biology, Indiana University, 6Department of Biological Sciences, University of Notre Dame

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Summary
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Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.

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Keywords: Bacterial Surface Motility Swarming Swarm Plate Assay Agar Concentration Wettability Liquid-solid-air Interface Fluorescent/bioluminescent Reporters Time-lapse Imaging Quantification Competing Swarming Species
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