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Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein
 

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Article DOI: 10.3791/52404-v • 09:49 min • April 2nd, 2015
April 2nd, 2015 •
1, 1
1School of Biological Sciences, Nanyang Technological University

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Summary
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Sedimentation equilibrium (SE) can be used to study protein-protein interactions in a physiological environment. This manuscript describes the use of this technique to determine the effect of pH on the stability of a homo-pentamer formed by the small hydrophobic (SH) protein encoded by the human syncytial respiratory virus (hRSV).

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Keywords: Sedimentation Equilibrium Analytical Ultracentrifugation Membrane Protein Oligomer Pentamer Histidine Protonation Protein-protein Interaction Association Constant Stoichiometry Thermodynamics Respiratory Syncytial Virus SH Protein
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