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Anvendelse af et High-throughput<em> In vitro</em> Mikrofluidsystem at udvikle Oral Multi-arter biofilm
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Chapters
- 00:05Title
- 02:06Preparation of Pooled Human Saliva as a Bacteria-positive Inoculum
- 02:51Growth and Live-dead Staining of Biofilms in a Microfluidic Chip
- 05:57Results: Live-dead Staining of Oral Cavity Biofilms Grown in Microfluidic Chips
- 06:43Conclusion
Målet med denne metoder papir er at beskrive anvendelsen af et mikrofluidsystem for udviklingen af multi-arter biofilm, der indeholder arter, typisk identificeret i human supragingival plak. Metoder til at beskrive biofilm arkitektur, biofilm levedygtighed, og en tilgang til høst biofilm for kultur-afhængige eller kultur-uafhængige analyser fremhævet.
Tags
High-throughputIn VitroMicrofluidic SystemMulti-species BiofilmsOral BiofilmsHuman SalivaSupragingival Dental PlaqueConfocal Laser Scanning Microscopy3D Biofilm ReconstructionBiofilm ArchitectureBiofilm ViabilityStreptococcusNeisseriaVeillonellaGemellaPorphyromonasBiofilm Cell HarvestingDNA ExtractionData Analysis
