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En guide til generering og brug hiPSC Afledte NPC'ere for Studiet af neurologiske sygdomme
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JoVE Journal Medicine
A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases

En guide til generering og brug hiPSC Afledte NPC'ere for Studiet af neurologiske sygdomme

DOI:
10.3791/52495-v

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09:30 min

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February 21, 2015

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, ,
1Department of Psychiatry,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:05Title
  • 01:22Harvest of Neural Rosettes
  • 03:03Expansion of Neural Progenitor Cells
  • 04:27NPC Transduction
  • 05:15Neurosphere Migration Assay
  • 07:06Results: Patient-specific hiPSCs can be Differentiated into Healthy NPCs and Neurons
  • 08:56Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how neural progenitor cells can be differentiated from human induced pluripotent stem cells, in order to yield a robust and replicative neural cell population, which may be used to identify the developmental pathways contributing to disease pathogenesis in many neurological disorders.

Tags

HiPSCNeural Progenitor CellsNeurological DiseasesPost-mortem StudiesDisease InitiationPatient-derivedDifferentiationNeurospheresGenetic ScreeningCellular Phenotypes

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