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Primary Tumor and MEF Cell Isolation to Study Lung Metastasis
 

Primary Tumor and MEF Cell Isolation to Study Lung Metastasis

Article DOI: 10.3791/52609-v • 06:31 min • May 20th, 2015
May 20th, 2015 •
*1, *1, 1
1Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center
* These authors contributed equally

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Summary
Automatic Translation

The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.

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Keywords: Primary Tumor MEF Cell Isolation Lung Metastasis Breast Tumorigenesis Metastatic Stage Genetically Engineered Mouse Model Mouse Embryonic Fibroblasts Cancer Cell Gene Expression Signaling Mechanisms Embryonic Abnormalities
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