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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
 

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Article DOI: 10.3791/52620-v • 08:37 min • March 30th, 2015
March 30th, 2015 •
*1, *1, 1
1Department of Bioengineering, Rice University
* These authors contributed equally

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Summary
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Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.

Tags

DNA Quantification Recombinase Polymerase Amplification (RPA) Quantitative RPA (qRPA) Assay Internal Positive Control (IPC) Isothermal Amplification HIV-1 DNA Point-of-care Diagnostics
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