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Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
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Immunology and Infection
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JoVE Journal Immunology and Infection
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

DOI:
10.3791/52666-v

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07:32 min

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March 19, 2015

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, , ,
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC),Clemson University, 2Department of Biology,Western Carolina University

Chapters

  • 00:05Title
  • 01:07DNA Preparation
  • 02:41Gene Gun Operation
  • 04:59Plating Transformed Cells
  • 05:52Results: Successful Homologous Recombination
  • 07:04Conclusion

Summary

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Automatic Translation

Biolistic transformation is a method used to generate stable integration of DNA into the genome of the opportunistic pathogen Cryptococcus neoformans through homologous recombination. We will demonstrate biolistic transformation of a construct, which has the gene encoding acetate kinase fused to the fluorescent tag mCherry into C. neoformans.

Tags

Keywords: Biolistic TransformationFluorescent Tagged GeneCryptococcus NeoformansOpportunistic Fungal PathogenAcetate ProductionXylulose-5-phosphate/fructose-6-phosphate PhosphoketolaseAcetate KinaseMCherryHomologous RecombinationFungal Meningitis

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